AJ Malcolm, PM Logan, RC Shipman, R Kurth, JG Levy; Analysis of human myelogenous leukemia cells in the fluorescence- activated cell sorter using a tumor-specific antiserum.
Green fluorescent protein (GFP) is widely used as a reporter gene in both prokaryotes and eukaryotes. However, the fluorescence levels of wild-type GFP (wtGFP) are not bright enough for fluorescence-activated cell sorting or flow cytometry. Several GFP variants were generated that are brighter or have altered excitation spectra when expressed in prokaryotic cells. We engineered two GFP genes.